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EZBioscience是ZScience Biotechnology Corporation Limited是美國(guó)的一個(gè)生物技術(shù)品牌，主要專(zhuān)注于新技術(shù)開(kāi)發(fā)和制造用于分子生物學(xué)的智能工具。EZBioscience的科學(xué)家和工程師是蛋白質(zhì)表達(dá)、純化、結(jié)晶、結(jié)構(gòu)測(cè)定和化學(xué)合成方面的專(zhuān)家，在分子克隆、RNA純化和相關(guān)檢測(cè)領(lǐng)域也擁有高度復(fù)雜的技術(shù)體系。EZBioscience的使命是通過(guò)向世界各地的科學(xué)家提供設(shè)計(jì)更巧妙的試劑和試劑盒，幫助使生物和醫(yī)學(xué)研究更容易。EZBioscience對(duì)醫(yī)療研究人員的承諾是以?xún)?yōu)惠的價(jià)格提供優(yōu)質(zhì)的產(chǎn)品。EZBioscience，使研究更容易。
EZBioscience熱銷(xiāo)產(chǎn)品
產(chǎn)品介紹：The EZBioscience® EZ-press SINGLE Cell to cDNA Kit allows researchers to analyze the gene expression of single or very low cell numbers (1 ~ 1,000 cells) in a reliable, sensitive and easy way. Cells are first lysed by the unique Cell Lysis Buffer. Then cDNA is produced and pre-amplified using gene specific primers from the cell lysate in the same tube. Due to the pre-amplification following reverse transcription, extremely low concentration of RNA from a very small amount of cells can be detected by the subsequent qPCR.
產(chǎn)品介紹：The EZBioscience® EZ-press RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of sources, without the need for toxic substances such as phenol or chloroform. The kit can be used with cultured cells. Biological samples are first lysed and homogenized in a strong denaturant containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. After homogenization, ethanol is added to the lysate, creating conditions that promote selective binding of RNA to the silica-based membrane. The sample is then applied to the Spin Column, where the total RNA binds to the membrane, then the membrane is treated with gDNA Remover to eliminate the DNA. Contaminants are efficiently washed away by subsequent step of washing. The high-quality total RNA is then eluted in Elution Buffer. The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, and so on.
產(chǎn)品介紹：TheEZBioscience®4× EZscript Reverse Transcription Mix II (with gDNA Remover) is a new-generation fast reverse transcription kit with higher reverse transcription efficiency compared to previous generations.
The kit is suitable for real-time RT-PCR (RT-qPCR) that contains a gDNA Remover which can effectively eliminate the contamination of genomic DNA (gDNA) or other double stranded DNA during the analysis of gene expression. To accurately analyze gene expression, it is necessary to detect cDNA in samples without contaminating DNA. To avoid amplification of gDNA, primers can be designed on different exons spanning introns. However, there may be cases where a suitable primer cannot be designed, as with a gene with a single exon or a gene without a long intron. Also, it may be difficult to avoid unexpected amplification from gDNA due to non-specific amplification or the existence of pseudo-genes. Moreover, some labs are heavily contaminated by the PCR products of previous tests. This kit offers a potent gDNA Remover that can eliminate double stranded DNA in RNA sample in 5 minutes at room temperature without loss of RNA. Then the first strand of cDNA is synthesized by adding the 4× EZscript RT Mix II. Reaction products are applicable to subsequent PCR, qPCR.
The mix contains reverse transcriptase, RNase Inhibitor, optimized buffer system, dNTPs, and Oligo dT18 and Random Hexamer as primers. The reverse transcriptase in this Mix is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. And the multiple site-mutations of reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. This product is also very suitable for reverse transcription using plant RNA.
EZBioscience代理——北京百奧創(chuàng)新科技有限公司，提供EZBioscience全系列產(chǎn)品。EZBioscience華中代理，EZBioscience華北代理，EZBioscience華南代理，EZBioscience華東代理。北京百奧創(chuàng)新科技有限公司作為一家專(zhuān)注于免疫學(xué)、細(xì)胞生物學(xué)、分子生物學(xué)等領(lǐng)域核心試劑產(chǎn)品的研發(fā)、生產(chǎn)與銷(xiāo)售的高新企業(yè)。北京百奧創(chuàng)新科技有限公司是中關(guān)村高新技術(shù)企業(yè)，于2017年在北京成立。百奧創(chuàng)新依托于中科院的科研技術(shù)力量，以中科院資深科學(xué)家為核心，整合經(jīng)驗(yàn)豐富的商業(yè)團(tuán)隊(duì)及管理團(tuán)隊(duì)組建而成。百奧創(chuàng)新在產(chǎn)品自主研發(fā)的同時(shí)，也與全球多家生命科學(xué)試劑供應(yīng)商建立良好的合作關(guān)系，基于“客戶(hù)第一"的核心理念，向全國(guó)10000+客戶(hù)提供200000+優(yōu)質(zhì)產(chǎn)品。百奧創(chuàng)新將以品質(zhì)樹(shù)立品牌，以服務(wù)贏得口碑，致力于成長(zhǎng)為重要的生物原料及生物化學(xué)試劑開(kāi)發(fā)與成果轉(zhuǎn)化的開(kāi)TUO者，并成為行業(yè)的優(yōu)質(zhì)供應(yīng)商。
